1NE9

Crystal Structure of Weissella viridescens FemX at 1.7 Ang Resolution

1NE9 の概要

• エントリーDOI: 10.2210/pdb1ne9/pdb
• 分子名称: FemX, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: protein, ligase
• 由来する生物種: Weissella viridescens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 38268.72

構造登録者

Biarrotte-Sorin, S.,Maillard, A.P.,Delettre, J.,Sougakoff, W.,Arthur, M.,Mayer, C. (登録日: 2002-12-11, 公開日: 2004-02-10, 最終更新日: 2024-02-14)

主引用文献

Biarrotte-Sorin, S.,Maillard, A.P.,Delettre, J.,Sougakoff, W.,Arthur, M.,Mayer, C.
Crystal structures of Weissella viridescens FemX and its complex with UDP-MurNAc-pentapeptide: insights into FemABX family substrates recognition.
Structure, 12:257-267, 2004

PubMed Abstract: Members of the FemABX protein family are novel therapeutic targets, as they are involved in the synthesis of the bacterial cell wall. They catalyze the addition of amino acid(s) on the peptidoglycan precursor using aminoacylated tRNA as a substrate. We report here the high-resolution structure of Weissella viridescens L-alanine transferase FemX and its complex with the UDP-MurNAc-pentapeptide. This is the first structure example of a FemABX family member that does not possess a coiled-coil domain. FemX consists of two structurally equivalent domains, separated by a cleft containing the binding site of the UDP-MurNAc-pentapeptide and a long channel that traverses one of the two domains. Our structural studies bring new insights into the evolution of the FemABX and the related GNAT superfamilies, shed light on the recognition site of the aminoacylated tRNA in Fem proteins, and allowed manual docking of the acceptor end of the alanyl-tRNAAla.

PubMed: 14962386
DOI: 10.1016/j.str.2004.01.006

実験手法

X-RAY DIFFRACTION (1.7 Å)