1NAG

CREVICE-FORMING MUTANTS IN THE RIGID CORE OF BOVINE PANCREATIC TRYPSIN INHIBITOR: CRYSTAL STRUCTURES OF F22A, Y23A, N43G, AND F45A

1NAG の概要

• エントリーDOI: 10.2210/pdb1nag/pdb
• 分子名称: BOVINE PANCREATIC TRYPSIN INHIBITOR, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: serine protease inhibitor
• 由来する生物種: Bos taurus (cattle)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6565.49

構造登録者

Danishefsky, A.T.,Wlodawer, A.,Kim, K.-S.,Tao, F.,Woodward, C. (登録日: 1992-08-18, 公開日: 1993-10-31, 最終更新日: 2024-10-30)

主引用文献

Danishefsky, A.T.,Housset, D.,Kim, K.S.,Tao, F.,Fuchs, J.,Woodward, C.,Wlodawer, A.
Crevice-forming mutants in the rigid core of bovine pancreatic trypsin inhibitor: crystal structures of F22A, Y23A, N43G, and F45A.
Protein Sci., 2:577-587, 1993

PubMed Abstract: Crystal structures of four mutants of bovine pancreatic trypsin inhibitor (F22A, Y23A, N43G, and F45A), engineered to alter their stability properties, have been determined. The mutated residues, which are highly conserved among Kunitz-type inhibitors, are located in the rigid core of the molecule. Replacement of the partially buried bulky residues of the wild-type protein with smaller residues resulted in crevices open to the exterior of the molecule. The overall three-dimensional structure of these mutants is very similar to that of the wild-type protein and only small rearrangements are observed among the atoms lining the crevices.

PubMed: 8518731

実験手法

X-RAY DIFFRACTION (1.9 Å)