1NA3

Design of Stable alpha-Helical Arrays from an Idealized TPR Motif

1NA3 の概要

• エントリーDOI: 10.2210/pdb1na3/pdb
• 関連するPDBエントリー: 1NA0
• 分子名称: designed protein CTPR2, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, 1-methylethyl 1-thio-beta-D-galactopyranoside, ... (5 entities in total)
• 機能のキーワード: design, tpr, de novo protein
• 由来する生物種: unidentified
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 21351.33

構造登録者

Main, E.,Xiong, Y.,Cocco, M.,D'Andrea, L.,Regan, L. (登録日: 2002-11-26, 公開日: 2003-06-03, 最終更新日: 2024-02-14)

主引用文献

Main, E.,Xiong, Y.,Cocco, M.,D'Andrea, L.,Regan, L.
Design of Stable alpha-Helical Arrays from an Idealized TPR Motif
Structure, 11:497-508, 2003

PubMed Abstract: The tetratricopeptide repeat (TPR) is a 34-amino acid alpha-helical motif that occurs in over 300 different proteins. In the different proteins, three to sixteen or more TPR motifs occur in tandem arrays and function to mediate protein-protein interactions. The binding specificity of each TPR protein is different, although the underlying structural motif is the same. Here we describe a statistical approach to the design of an idealized TPR motif. We present the high-resolution X-ray crystal structures (to 1.55 and 1.6 A) of designed TPR proteins and describe their solution properties and stability. A detailed analysis of these structures provides an understanding of the TPR motif, how it is repeated to give helical arrays with different superhelical twists, and how a very stable framework may be constructed for future functional designs.

PubMed: 12737816
DOI: 10.1016/S0969-2126(03)00076-5

実験手法

X-RAY DIFFRACTION (1.55 Å)