1NA1

The structure of HRV14 when complexed with Pleconaril

1NA1 の概要

• エントリーDOI: 10.2210/pdb1na1/pdb
• 分子名称: Coat protein VP1, Coat protein VP2, Coat protein VP3, ... (6 entities in total)
• 機能のキーワード: hrv14, human rhinovirus 14, pleconaril, icosahedral virus, virus
• 由来する生物種: Human rhinovirus 14; 詳細
• 細胞内の位置: Capsid protein VP0: Virion . Capsid protein VP4: Virion . Capsid protein VP2: Virion . Capsid protein VP3: Virion . Capsid protein VP1: Virion . Protein 2B: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Protein 2C: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Protein 3A: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Protein 3AB: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Viral protein genome-linked: Virion . Protease 3C: Host cytoplasm . Protein 3CD: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . RNA-directed RNA polymerase: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side : P03303 P03303 P03303 P03303
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 94863.88

構造登録者

Zhang, Y.,Simpson, A.A.,Bator, C.M.,Chakravarty, S.,Pevear, D.C.,Skochko, G.A.,Tull, T.M.,Diana, G.,Rossmann, M.G. (登録日: 2002-11-26, 公開日: 2003-12-16, 最終更新日: 2024-05-22)

主引用文献

Zhang, Y.,Simpson, A.A.,Ledford, R.M.,Bator, C.M.,Chakravarty, S.,Skochko, G.A.,Demenczuk, T.M.,Watanyar, A.,Pevear, D.C.,Rossmann, M.G.
Structural and virological studies of the stages of virus replication that are affected by antirhinovirus compounds
J.Virol., 78:11061-11069, 2004

PubMed Abstract: Pleconaril is a broad-spectrum antirhinovirus and antienterovirus compound that binds into a hydrophobic pocket within viral protein 1, stabilizing the capsid and resulting in the inhibition of cell attachment and RNA uncoating. When crystals of human rhinovirus 16 (HRV16) and HRV14 are incubated with pleconaril, drug occupancy in the binding pocket is lower than when pleconaril is introduced during assembly prior to crystallization. This effect is far more marked in HRV16 than in HRV14 and is more marked with pleconaril than with other compounds. These observations are consistent with virus yield inhibition studies and radiolabeled drug binding studies showing that the antiviral effect of pleconaril against HRV16 is greater on the infectivity of progeny virions than the parent input viruses. These data suggest that drug integration into the binding pocket during assembly, or at some other late stage in virus replication, may contribute to the antiviral activity of capsid binding compounds.

PubMed: 15452226
DOI: 10.1128/JVI.78.20.11061-11069.2004

実験手法

X-RAY DIFFRACTION (3.3 Å)