1N99

CRYSTAL STRUCTURE OF THE PDZ TANDEM OF HUMAN SYNTENIN

1N99 の概要

• エントリーDOI: 10.2210/pdb1n99/pdb
• 分子名称: Syntenin 1 (2 entities in total)
• 機能のキーワード: pdz domain, signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell junction, focal adhesion: O00560
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36412.54

構造登録者

Kang, B.S.,Cooper, D.R.,Jelen, F.,Devedjiev, Y.,Derewenda, U.,Dauter, Z.,Otlewski, J.,Derewenda, Z.S. (登録日: 2002-11-22, 公開日: 2003-04-15, 最終更新日: 2024-11-06)

主引用文献

Kang, B.S.,Cooper, D.R.,Jelen, F.,Devedjiev, Y.,Derewenda, U.,Dauter, Z.,Otlewski, J.,Derewenda, Z.S.
PDZ Tandem of Human Syntenin: Crystal Structure and Functional Properties
Structure, 11:459-468, 2003

PubMed Abstract: Syntenin, a 33 kDa protein, interacts with several cell membrane receptors and with merlin, the product of the causal gene for neurofibromatosis type II. We report a crystal structure of the functional fragment of human syntenin containing two canonical PDZ domains, as well as binding studies for full-length syntenin, the PDZ tandem, and isolated PDZ domains. We show that the functional properties of syntenin are a result of independent interactions with target peptides, and that each domain is able to bind peptides belonging to two different classes: PDZ1 binds peptides from classes I and III, while PDZ2 interacts with classes I and II. The independent binding of merlin by PDZ1 and syndecan-4 by PDZ2 provides direct evidence for the coupling of syndecan-mediated signaling to actin regulation by merlin.

PubMed: 12679023
DOI: 10.1016/S0969-2126(03)00052-2

実験手法

X-RAY DIFFRACTION (1.94 Å)