1N59

Crystal structure of the Murine class I Major Histocompatibility Complex of H-2KB, B2-Microglobulin, and A 9-Residue immunodominant peptide epitope gp33 derived from LCMV

1N59 の概要

• エントリーDOI: 10.2210/pdb1n59/pdb
• 分子名称: H-2 class I histocompatibility antigen, K-B alpha chain, Beta-2-microglobulin, nonameric peptide, gp33 derived from lymphocytic choriomeningitis virus, ... (4 entities in total)
• 機能のキーワード: murine mhc, viral escape, lcmv, immunodominant epitope, immune system
• 由来する生物種: Mus musculus (house mouse); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P01901; Secreted: P01887
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 89248.28

構造登録者

Achour, A.,Michaelsson, J.,Harris, R.A.,Odeberg, J.,Grufman, P.,Sandberg, J.K.,Levitsky, V.,Karre, K.,Sandalova, T.,Schneider, G. (登録日: 2002-11-05, 公開日: 2003-01-07, 最終更新日: 2024-10-30)

主引用文献

Achour, A.,Michaelsson, J.,Harris, R.A.,Odeberg, J.,Grufman, P.,Sandberg, J.K.,Levitsky, V.,Karre, K.,Sandalova, T.,Schneider, G.
A Structural Basis for LCMV Immune Evasion. Subversion of H-2D(b) and H-2K(b) Presentation of gp33 Revealed by Comparative Crystal Structure Analyses.
Immunity, 17:757-768, 2002

PubMed Abstract: LCMV infection of H-2(b) mice generates a CD8(+) CTL response mainly directed toward three immunodominant epitopes. One of these, gp33, is presented by both H-2D(b) and H-2K(b) MHC class I molecules. The virus can escape immune recognition in the context of both these MHC class I molecules through single mutations of the peptide. In order to understand the underlying structural mechanism, we determined the crystal structures of both complexes. The structures reveal that the peptide is presented in two diametrically opposed manners by H-2D(b) and H-2K(b), with residues used as anchor positions in one MHC class I molecule interacting with the TCR in the other. Importantly, the peptide's N-terminal residue p1K protrudes from the binding cleft in H-2K(b). We present structural evidence that explains the functional consequences of single mutations found in escape variants.

PubMed: 12479822
DOI: 10.1016/S1074-7613(02)00478-8

実験手法

X-RAY DIFFRACTION (2.95 Å)