1N3H

Coupling of Folding and Binding in the PTB Domain of the Signaling Protein Shc

Summary for 1N3H

• Entry DOI: 10.2210/pdb1n3h/pdb
• Related: 1SHC
• NMR Information: BMRB: 5566
• Descriptor: SHC Transforming protein (1 entity in total)
• Functional Keywords: free protein, beta sandwich, signaling protein
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm. Isoform p46Shc: Mitochondrion matrix. Isoform p66Shc: Mitochondrion (By similarity): P29353
• Total number of polymer chains: 1
• Total formula weight: 22657.02

Authors

Farooq, A.,Zeng, L.,Yan, K.S.,Ravichandran, K.S.,Zhou, M.-M. (deposition date: 2002-10-28, release date: 2003-10-28, Last modification date: 2024-05-22)

Primary citation

Farooq, A.,Zeng, L.,Yan, K.S.,Ravichandran, K.S.,Zhou, M.-M.
Coupling of Folding and Binding in the PTB Domain of the Signaling Protein Shc
Structure, 11:905-913, 2003

PubMed Abstract: The notion that certain proteins lack intrinsic globular structure under physiological conditions and that the attainment of fully folded structure only occurs upon the binding of target molecules has been recently gaining popularity. We report here the solution structure of the PTB domain of the signaling protein Shc in the free form. Comparison of this structure with that of the complex form, obtained previously with a phosphopeptide ligand, reveals that the Shc PTB domain is structurally disordered in the free form, particularly around the regions constituting the peptide binding pocket. The binding of the ligand appears to reorganize this pocket through local folding events triggering a conformational switch between the free and the complex forms.

PubMed: 12906822
DOI: 10.1016/S0969-2126(03)00134-5

Experimental method

SOLUTION NMR