1N3H

Coupling of Folding and Binding in the PTB Domain of the Signaling Protein Shc

1N3H の概要

• エントリーDOI: 10.2210/pdb1n3h/pdb
• 関連するPDBエントリー: 1SHC
• NMR情報: BMRB: 5566
• 分子名称: SHC Transforming protein (1 entity in total)
• 機能のキーワード: free protein, beta sandwich, signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm. Isoform p46Shc: Mitochondrion matrix. Isoform p66Shc: Mitochondrion (By similarity): P29353
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22657.02

構造登録者

Farooq, A.,Zeng, L.,Yan, K.S.,Ravichandran, K.S.,Zhou, M.-M. (登録日: 2002-10-28, 公開日: 2003-10-28, 最終更新日: 2024-05-22)

主引用文献

Farooq, A.,Zeng, L.,Yan, K.S.,Ravichandran, K.S.,Zhou, M.-M.
Coupling of Folding and Binding in the PTB Domain of the Signaling Protein Shc
Structure, 11:905-913, 2003

PubMed Abstract: The notion that certain proteins lack intrinsic globular structure under physiological conditions and that the attainment of fully folded structure only occurs upon the binding of target molecules has been recently gaining popularity. We report here the solution structure of the PTB domain of the signaling protein Shc in the free form. Comparison of this structure with that of the complex form, obtained previously with a phosphopeptide ligand, reveals that the Shc PTB domain is structurally disordered in the free form, particularly around the regions constituting the peptide binding pocket. The binding of the ligand appears to reorganize this pocket through local folding events triggering a conformational switch between the free and the complex forms.

PubMed: 12906822
DOI: 10.1016/S0969-2126(03)00134-5

実験手法

SOLUTION NMR