1N11

D34 REGION OF HUMAN ANKYRIN-R AND LINKER

1N11 の概要

• エントリーDOI: 10.2210/pdb1n11/pdb
• 分子名称: Ankyrin, BROMIDE ION, CHLORIDE ION (3 entities in total)
• 機能のキーワード: ankyrin, clathrin, band 3, anion exchanger, structural protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Isoform Er1: Cytoplasm, cytoskeleton. Isoform Mu17: Membrane. Isoform Mu18: Sarcoplasmic reticulum (Probable). Isoform Mu19: Sarcoplasmic reticulum (Probable). Isoform Mu20: Sarcoplasmic reticulum (Probable): P16157
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 46858.88

構造登録者

Michaely, P.,Tomchick, D.R.,Machius, M.,Anderson, R.G.W. (登録日: 2002-10-16, 公開日: 2002-12-11, 最終更新日: 2024-02-14)

主引用文献

Michaely, P.,Tomchick, D.R.,Machius, M.,Anderson, R.G.W.
Crystal structure of a 12 ANK repeat stack from human ankyrinR
Embo J., 21:6387-6396, 2002

PubMed Abstract: Ankyrins are multifunctional adaptors that link specific proteins to the membrane-associated, spectrin- actin cytoskeleton. The N-terminal, 'membrane-binding' domain of ankyrins contains 24 ANK repeats and mediates most binding activities. Repeats 13-24 are especially active, with known sites of interaction for the Na/K ATPase, Cl/HCO(3) anion exchanger, voltage-gated sodium channel, clathrin heavy chain and L1 family cell adhesion molecules. Here we report the crystal structure of a human ankyrinR construct containing ANK repeats 13-24 and a portion of the spectrin-binding domain. The ANK repeats are observed to form a contiguous spiral stack with which the spectrin-binding domain fragment associates as an extended strand. The structural information has been used to construct models of all 24 repeats of the membrane-binding domain as well as the interactions of the repeats with the Cl/HCO(3) anion exchanger and clathrin. These models, together with available binding studies, suggest that ion transporters such as the anion exchanger associate in a large central cavity formed by the ANK repeat spiral, while clathrin and cell adhesion molecules associate with specific regions outside this cavity.

PubMed: 12456646
DOI: 10.1093/emboj/cdf651

実験手法

X-RAY DIFFRACTION (2.7 Å)