1MWU

Structure of methicillin acyl-Penicillin binding protein 2a from methicillin resistant Staphylococcus aureus strain 27r at 2.60 A resolution.

1MWU の概要

• エントリーDOI: 10.2210/pdb1mwu/pdb
• 関連するPDBエントリー: 1MWR 1MWS 1MWT 1MWX
• 分子名称: penicillin-binding protein 2a, (2R,4S)-2-[(1R)-1-{[(2,6-dimethoxyphenyl)carbonyl]amino}-2-oxoethyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid, CADMIUM ION, ... (5 entities in total)
• 機能のキーワード: penicillin binding protein, beta-lactam, d, d-transpeptidase, d-carboxypeptidase, methicillin, biosynthetic protein
• 由来する生物種: Staphylococcus aureus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 149023.23

構造登録者

Lim, D.C.,Strynadka, N.C.J. (登録日: 2002-10-01, 公開日: 2002-11-06, 最終更新日: 2012-02-15)

主引用文献

Lim, D.,Strynadka, N.C.
Structural basis for the beta lactam resistance of PBP2a from methicillin-resistant Staphylococcus aureus.
Nat.Struct.Biol., 9:870-876, 2002

PubMed Abstract: The multiple antibiotic resistance of methicillin-resistant strains of Staphylococcus aureus (MRSA) has become a major clinical problem worldwide. The key determinant of the broad-spectrum beta-lactam resistance in MRSA strains is the penicillin-binding protein 2a (PBP2a). Because of its low affinity for beta-lactams, PBP2a provides transpeptidase activity to allow cell wall synthesis at beta-lactam concentrations that inhibit the beta-lactam-sensitive PBPs normally produced by S. aureus. The crystal structure of a soluble derivative of PBP2a has been determined to 1.8 A resolution and provides the highest resolution structure for a high molecular mass PBP. Additionally, structures of the acyl-PBP complexes of PBP2a with nitrocefin, penicillin G and methicillin allow, for the first time, a comparison of an apo and acylated resistant PBP. An analysis of the PBP2a active site in these forms reveals the structural basis of its resistance and identifies features in newly developed beta-lactams that are likely important for high affinity binding.

PubMed: 12389036

実験手法

X-RAY DIFFRACTION (2.6 Å)