1MWE

THE X-RAY STRUCTURE OF A COMPLEX OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE COMPLEXED WITH SIALIC ACID AT 4 DEGREES C REVEALING A SECOND SIALIC ACID BINDING SITE

1MWE の概要

• エントリーDOI: 10.2210/pdb1mwe/pdb
• 分子名称: NEURAMINIDASE, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: hydrolase, neuraminidase, avian influenza, sialic acid binding, hemagglutination site, heme absorbing site
• 由来する生物種: Influenza A virus
• 細胞内の位置: Virion membrane (By similarity): P03472
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 46645.48

構造登録者

Varghese, J.N. (登録日: 1997-08-27, 公開日: 1998-03-04, 最終更新日: 2024-11-13)

主引用文献

Varghese, J.N.,Colman, P.M.,van Donkelaar, A.,Blick, T.J.,Sahasrabudhe, A.,McKimm-Breschkin, J.L.
Structural evidence for a second sialic acid binding site in avian influenza virus neuraminidases.
Proc.Natl.Acad.Sci.USA, 94:11808-11812, 1997

PubMed Abstract: The x-ray structure of a complex of sialic acid (Neu5Ac) with neuraminidase N9 subtype from A/tern/Australia/G70C/75 influenza virus at 4 degrees C has revealed the location of a second Neu5Ac binding site on the surface of the enzyme. At 18 degrees C, only the enzyme active site contains bound Neu5Ac. Neu5Ac binds in the second site in the chair conformation in a similar way to which it binds to hemagglutinin. The residues that interact with Neu5Ac at this second site are mostly conserved in avian strains, but not in human and swine strains, indicating that it has some as-yet-unknown biological function in birds.

PubMed: 9342319
DOI: 10.1073/pnas.94.22.11808

実験手法

X-RAY DIFFRACTION (1.7 Å)