1MW4

Solution structure of the human Grb7-SH2 domain in complex with a 10 amino acid peptide pY1139

1MW4 の概要

• エントリーDOI: 10.2210/pdb1mw4/pdb
• 分子名称: Growth factor receptor-bound protein 7, Receptor protein-tyrosine kinase erbB-2 (2 entities in total)
• 機能のキーワード: sh2 domain in complex with a ligand, hormone-growth factor-transferase complex, hormone/growth factor/transferase
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: Q14451; Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cytoplasm. Isoform 3: Cytoplasm: P04626
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 14956.92

構造登録者

Ivancic, M.,Lyons, B.A. (登録日: 2002-09-27, 公開日: 2003-09-09, 最終更新日: 2024-10-16)

主引用文献

Ivancic, M.,Daly, R.J.,Lyons, B.A.
Solution structure of the human Grb7-SH2 domain/erbB2 peptide complex and structural basis for Grb7 binding to ErbB2
J.BIOMOL.NMR, 27:205-219, 2003

PubMed Abstract: The solution structure of the hGrb7-SH2 domain in complex with a ten amino acid phosphorylated peptide ligand representative of the erbB2 receptor tyrosine kinase (pY1139) is presented as determined by nuclear magnetic resonance methods. The hGrb7-SH2 domain structure reveals the Src homology 2 domain topology consisting of a central beta-sheet capped at each end by an alpha-helix. The presence of a four residue insertion in the region between beta-strand E and the EF loop and resulting influences on the SH2 domain/peptide complex structure are discussed. The binding conformation of the erbB2 peptide is in a beta-turn similar to that found in phosphorylated tyrosine peptides bound to the Grb2-SH2 domain. To our knowledge this is only the second example of an SH2 domain binding its naturally occurring ligands in a turn, instead of extended, conformation. Close contacts between residues responsible for binding specificity in hGrb7-SH2 and the erbB2 peptide are characterized and the potential effect of mutation of these residues on the hGrb7-SH2 domain structure is discussed.

PubMed: 12975581
DOI: 10.1023/A:1025498409113

実験手法

SOLUTION NMR