1MSW

Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase

1MSW の概要

• エントリーDOI: 10.2210/pdb1msw/pdb
• 分子名称: Template DNA, Non-Template DNA, RNA message, ... (5 entities in total)
• 機能のキーワード: t7rnap elongation complex, transcription-dna-rna complex, transcription/dna/rna
• 由来する生物種: Enterobacteria phage T7; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 113535.54

構造登録者

Yin, Y.W.,Steitz, T.A. (登録日: 2002-09-19, 公開日: 2002-11-15, 最終更新日: 2024-02-14)

主引用文献

Yin, Y.W.,Steitz, T.A.
Structural Basis for the Transition from Initiation to Elongation Transcription in T7 RNA Polymerase
Science, 298:1387-1395, 2002

PubMed Abstract: To make messenger RNA transcripts, bacteriophage T7 RNA polymerase (T7 RNAP) undergoes a transition from an initiation phase, which only makes short RNA fragments, to a stable elongation phase. We have determined at 2.1 angstrom resolution the crystal structure of a T7 RNAP elongation complex with 30 base pairs of duplex DNA containing a "transcription bubble" interacting with a 17-nucleotide RNA transcript. The transition from an initiation to an elongation complex is accompanied by a major refolding of the amino-terminal 300 residues. This results in loss of the promoter binding site, facilitating promoter clearance, and creates a tunnel that surrounds the RNA transcript after it peels off a seven-base pair heteroduplex. Formation of the exit tunnel explains the enhanced processivity of the elongation complex. Downstream duplex DNA binds to the fingers domain, and its orientation relative to upstream DNA in the initiation complex implies an unwinding that could facilitate formation of the open promoter complex.

PubMed: 12242451
DOI: 10.1126/science.1077464

実験手法

X-RAY DIFFRACTION (2.1 Å)