1MME

THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE

1MME の概要

• エントリーDOI: 10.2210/pdb1mme/pdb
• 分子名称: RNA HAMMERHEAD RIBOZYME (2 entities in total)
• 機能のキーワード: rna hammerhead ribozyme, catalytic rna, loop, ribozyme
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 26379.96

構造登録者

Scott, W.G.,Finch, J.T.,Klug, A. (登録日: 1995-12-09, 公開日: 1996-02-06, 最終更新日: 2024-02-14)

主引用文献

Scott, W.G.,Finch, J.T.,Klug, A.
The crystal structure of an all-RNA hammerhead ribozyme: a proposed mechanism for RNA catalytic cleavage.
Cell(Cambridge,Mass.), 81:991-1002, 1995

PubMed Abstract: We have solved the crystal structure of an all-RNA hammerhead ribozyme having a single 2'-O-methyl cytosine incorporated at the active site to prevent cleavage. The conditions used differ from those in another recent solution in four significant ways: first, it is an all-RNA ribozyme rather than a DNA-RNA hybrid; second, the connectivity of the ribozyme backbone strands is different; third, the crystals were grown in the presence of a much lower concentration of salt; and fourth, the crystal packing scheme is very different. Nevertheless, the three-dimensional structure of the all-RNA hammerhead ribozyme is similar to the previous structure. Five potential Mg(II)-binding sites are identified, including one positioned near the ribozyme catalytic pocket. Upon this basis, as well as upon comparisons with the metal-binding sites in the structurally homologous uridine turn of tRNAPhe, we propose a mechanism for RNA catalytic cleavage.

PubMed: 7541315
DOI: 10.1016/S0092-8674(05)80004-2

実験手法

X-RAY DIFFRACTION (3.1 Å)