1ML0

VIRAL CHEMOKINE BINDING PROTEIN M3 FROM MURINE GAMMAHERPESVIRUS68 IN COMPLEX WITH THE P8A VARIANT OF CC-CHEMOKINE MCP-1

1ML0 の概要

• エントリーDOI: 10.2210/pdb1ml0/pdb
• 関連するPDBエントリー: 1DOK 1MKF
• 分子名称: M3 Protein, Small Inducible Cytokine (3 entities in total)
• 機能のキーワード: herpesvirus, viral immune evasion, chemokine binding protein, decoy receptor, immune system
• 由来する生物種: Murid herpesvirus 4 (Murine herpesvirus 68); 詳細
• 細胞内の位置: Secreted: P13500
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50481.20

構造登録者

Alexander, J.M.,Fremont, D.H. (登録日: 2002-08-29, 公開日: 2002-11-13, 最終更新日: 2024-11-06)

主引用文献

Alexander, J.M.,Nelson, C.A.,van Berkel, V.,Lau, E.K.,Studts, J.M.,Brett, T.J.,Speck, S.H.,Handel, T.M.,Virgin, H.W.,Fremont, D.H.
Structural Basis of Chemokine Sequestration by a Herpesvirus Decoy Receptor
Cell(Cambridge,Mass.), 111:343-356, 2002

PubMed Abstract: The M3 protein encoded by murine gamma herpesvirus68 (gamma HV68) functions as an immune system saboteur by the engagement of chemoattractant cytokines, thereby altering host antiviral inflammatory responses. Here we report the crystal structures of M3 both alone and in complex with the CC chemokine MCP-1. M3 is a two-domain beta sandwich protein with a unique sequence and topology, forming a tightly packed anti-parallel dimer. The stoichiometry of the MCP-1:M3 complex is 2:2, with two monomeric chemokines embedded at distal ends of the preassociated M3 dimer. Conformational flexibility and electrostatic complementation are both used by M3 to achieve high-affinity and broad-spectrum chemokine engagement. M3 also employs structural mimicry to promiscuously sequester chemokines, engaging conservative structural elements associated with both chemokine homodimerization and binding to G protein-coupled receptors.

PubMed: 12419245
DOI: 10.1016/S0092-8674(02)01007-3

実験手法

X-RAY DIFFRACTION (2.8 Å)