1MK7

CRYSTAL STRUCTURE OF AN INTEGRIN BETA3-TALIN CHIMERA

1MK7 の概要

• エントリーDOI: 10.2210/pdb1mk7/pdb
• 関連するPDBエントリー: 1MIX 1MIZ 1MK9
• 分子名称: Integrin Beta3, TALIN (3 entities in total)
• 機能のキーワード: focal adhesion, integrin binding, ferm domain, cytoskeleton, npxy motif, ptb domain, structural protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P05106; Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side: P54939
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 47831.14

構造登録者

Garcia-Alvarez, B.,De Pereda, J.M.,Calderwood, D.A.,Ulmer, T.S.,Critchley, D.,Campbell, I.D.,Ginsberg, M.H.,Liddington, R.C. (登録日: 2002-08-28, 公開日: 2003-01-28, 最終更新日: 2024-10-16)

主引用文献

Garcia-Alvarez, B.,De Pereda, J.M.,Calderwood, D.A.,Ulmer, T.S.,Critchley, D.,Campbell, I.D.,Ginsberg, M.H.,Liddington, R.C.
Structural Determinants of Integrin Recognition by Talin
Mol.Cell, 11:49-58, 2003

PubMed Abstract: The binding of cytoplasmic proteins, such as talin, to the cytoplasmic domains of integrin adhesion receptors mediates bidirectional signal transduction. Here we report the crystal structure of the principal integrin binding and activating fragment of talin, alone and in complex with fragments of the beta 3 integrin tail. The FERM (four point one, ezrin, radixin, and moesin) domain of talin engages integrins via a novel variant of the canonical phosphotyrosine binding (PTB) domain-NPxY ligand interaction that may be a prototype for FERM domain recognition of transmembrane receptors. In combination with NMR and mutational analysis, our studies reveal the critical interacting elements of both talin and the integrin beta 3 tail, providing structural paradigms for integrin linkage to the cell interior.

PubMed: 12535520
DOI: 10.1016/S1097-2765(02)00823-7

実験手法

X-RAY DIFFRACTION (2.2 Å)