1MJS

MH2 domain of transcriptional factor SMAD3

1MJS の概要

• エントリーDOI: 10.2210/pdb1mjs/pdb
• 関連するPDBエントリー: 1MK2
• 分子名称: SMAD 3 (2 entities in total)
• 機能のキーワード: beta sandwich, transcription
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P84022
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22178.20

構造登録者

Qin, B.Y.,Lam, S.S.,Correia, J.J.,Lin, K. (登録日: 2002-08-28, 公開日: 2002-10-16, 最終更新日: 2024-04-03)

主引用文献

Qin, B.Y.,Lam, S.S.,Correia, J.J.,Lin, K.
Smad3 allostery links TGF-beta receptor kinase activation to transcriptional control
Genes Dev., 16:1950-1963, 2002

PubMed Abstract: Smad3 transduces the signals of TGF-betas, coupling transmembrane receptor kinase activation to transcriptional control. The membrane-associated molecule SARA (Smad Anchor for Receptor Activation) recruits Smad3 for phosphorylation by the receptor kinase. Upon phosphorylation, Smad3 dissociates from SARA and enters the nucleus, in which its transcriptional activity can be repressed by Ski. Here, we show that SARA and Ski recognize specifically the monomeric and trimeric forms of Smad3, respectively. Thus, trimerization of Smad3, induced by phosphorylation, simultaneously activates the TGF-beta signal by driving Smad3 dissociation from SARA and sets up the negative feedback mechanism by Ski. Structural models of the Smad3/SARA/receptor kinase complex and Smad3/Ski complex provide insights into the molecular basis of regulation.

PubMed: 12154125
DOI: 10.1101/gad.1002002

実験手法

X-RAY DIFFRACTION (1.91 Å)