1MJO

METHIONINE HOLOREPRESSOR MUTANT (Q44K) PLUS COREPRESSOR (S-ADENOSYL METHIONINE) COMPLEXED TO THE MINIMAL MET CONSENSUS OPERATOR WITH THE CENTRAL TA STEP MUTATED TO AT

1MJO の概要

• エントリーDOI: 10.2210/pdb1mjo/pdb
• 関連するPDBエントリー: 1MJ2 1MJM 1MJP 1MJQ
• 分子名称: CONSENSUS DNA OPERATOR DUPLEX WITH THE CENTRAL TA STEP MUTATED TO AT, METHIONINE REPRESSOR, S-ADENOSYLMETHIONINE, ... (5 entities in total)
• 機能のキーワード: transcription regulation, metj, methionine repressor, sheet-helix-helix, s-adenosyl methionine, dna, complex (transcription regulation-dna), transcription-dna complex, transcription/dna
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: P0A8U6
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 61387.82

構造登録者

Garvie, C.W.,Phillips, S.E.V. (登録日: 1998-01-28, 公開日: 1999-08-02, 最終更新日: 2023-08-02)

主引用文献

Garvie, C.W.,Phillips, S.E.
Direct and indirect readout in mutant Met repressor-operator complexes.
Structure Fold.Des., 8:905-914, 2000

PubMed Abstract: The methionine repressor, MetJ, represses the transcription of genes involved in methionine biosynthesis by binding to arrays of two to five adjacent copies of an eight base-pair 'metbox' sequence. Naturally occurring operators differ from the consensus sequence to a greater extent as the number of metboxes increases. MetJ, while accommodating this sequence variation in natural operators, is very sensitive to particular base changes, even where bases are not directly contacted in the crystal structure of a complex formed between the repressor and consensus operator.

PubMed: 10986458
DOI: 10.1016/S0969-2126(00)00182-9

実験手法

X-RAY DIFFRACTION (2.1 Å)