1MJG

CRYSTAL STRUCTURE OF BIFUNCTIONAL CARBON MONOXIDE DEHYDROGENASE/ACETYL-COA SYNTHASE(CODH/ACS) FROM MOORELLA THERMOACETICA (F. CLOSTRIDIUM THERMOACETICUM)

1MJG の概要

• エントリーDOI: 10.2210/pdb1mjg/pdb
• 関連するPDBエントリー: 1E2U 1E9V 1JJY 1JQK
• 分子名称: CARBON MONOXIDE DEHYDROGENASE BETA SUBUNIT, Carbon monoxide dehydrogenase alpha subunit, IRON/SULFUR CLUSTER, ... (8 entities in total)
• 機能のキーワード: carbon monoxide dehydrogenase(codh), acetyl-coa synthase (acs), nickel-iron-sulfur cluster (ni-fe-s), nickel-coopper-iron-sulfur (ni-cu-fe-s) cluster, hydrophobic co channel, substrate tunnel, helical domain, rossmann fold, electron transfer, clostridium thermoaceticum, wood-ljundahl pathway, oxidoreductase
• 由来する生物種: Moorella thermoacetica; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 624897.83

構造登録者

Doukov, T.I.,Iverson, T.M.,Seravalli, J.,Ragsdale, S.W.,Drennan, C.L. (登録日: 2002-08-27, 公開日: 2003-01-28, 最終更新日: 2024-11-20)

主引用文献

Doukov, T.I.,Iverson, T.M.,Seravalli, J.,Ragsdale, S.W.,Drennan, C.L.
A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase
Science, 298:567-572, 2002

PubMed Abstract: A metallocofactor containing iron, sulfur, copper, and nickel has been discovered in the enzyme carbon monoxide dehydrogenase/acetyl-CoA (coenzyme A) synthase from Moorella thermoacetica (f. Clostridium thermoaceticum). Our structure at 2.2 angstrom resolution reveals that the cofactor responsible for the assembly of acetyl-CoA contains a [Fe4S4] cubane bridged to a copper-nickel binuclear site. The presence of these three metals together in one cluster was unanticipated and suggests a newly discovered role for copper in biology. The different active sites of this bifunctional enzyme complex are connected via a channel, 138 angstroms long, that provides a conduit for carbon monoxide generated at the C-cluster on one subunit to be incorporated into acetyl-CoA at the A-cluster on the other subunit.

PubMed: 12386327
DOI: 10.1126/science.1075843

実験手法

X-RAY DIFFRACTION (2.2 Å)