1MFM

MONOMERIC HUMAN SOD MUTANT F50E/G51E/E133Q AT ATOMIC RESOLUTION

1MFM の概要

• エントリーDOI: 10.2210/pdb1mfm/pdb
• 分子名称: PROTEIN (COPPER,ZINC SUPEROXIDE DISMUTASE), ZINC ION, COPPER (II) ION, ... (6 entities in total)
• 機能のキーワード: oxidoreductase, superoxide acceptor, monomeric mutant
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm : P00441
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17044.01

構造登録者

Ferraroni, M.,Rypniewski, W.,Wilson, K.S.,Orioli, P.L.,Viezzoli, M.S.,Banci, L.,Bertini, I.,Mangani, S. (登録日: 1999-04-16, 公開日: 1999-04-21, 最終更新日: 2023-12-27)

主引用文献

Ferraroni, M.,Rypniewski, W.,Wilson, K.S.,Viezzoli, M.S.,Banci, L.,Bertini, I.,Mangani, S.
The crystal structure of the monomeric human SOD mutant F50E/G51E/E133Q at atomic resolution. The enzyme mechanism revisited.
J.Mol.Biol., 288:413-426, 1999

PubMed Abstract: The crystal structure of the engineered monomeric human Cu,ZnSOD triple mutant F50E/G51E/E133Q (Q133M2SOD) is reported at atomic resolution (1.02 A). This derivative has about 20 % of the wild-type activity. Crystals of Q133M2SOD have been obtained in the presence of CdCl2. The metal binding site is disordered, with both cadmium and copper ions simultaneously binding to the copper site. The cadmium (II) ions occupy about 45 % of the copper sites by binding the four histidine residues which ligate copper in the native enzyme, and two further water molecules to complete octahedral coordination. The copper ion is tri-coordinate, and the fourth histidine (His63) is detached from copper and bridges cadmium and zinc. X-ray absorption spectroscopy performed on the crystals suggests that the copper ion has undergone partial photoreduction upon exposure to the synchrotron light. The structure is also disordered in the disulfide bridge region of loop IV that is located at the subunit/subunit interface in the native SOD dimer. As a consequence, the catalytically relevant Arg143 residue is disordered. The present structure has been compared to other X-ray structures on various isoenzymes and to the solution structure of the same monomeric form. The structural results suggest that the low activity of monomeric SOD is due to the disorder in the conformation of the side-chain of Arg143 as well as of loop IV. It is proposed that the subunit-subunit interactions in the multimeric forms of the enzyme are needed to stabilize the correct geometry of the cavity and the optimal orientation of the charged residues in the active channel. Furthermore, the different coordination of cadmium and copper ions, contemporaneously present in the same site, are taken as models for the oxidized and reduced copper species, respectively. These properties of the structure have allowed us to revisit the enzymatic mechanism.

PubMed: 10329151
DOI: 10.1006/jmbi.1999.2681

実験手法

X-RAY DIFFRACTION (1.02 Å)