1MFE

RECOGNITION OF A CELL-SURFACE OLIGO-SACCHARIDE OF PATHOGENIC SALMONELLA BY AN ANTIBODY FAB FRAGMENT

Summary for 1MFE

• Entry DOI: 10.2210/pdb1mfe/pdb
• Descriptor: IGG1-LAMBDA SE155-4 FAB (LIGHT CHAIN), IGG1-LAMBDA SE155-4 FAB (HEAVY CHAIN), alpha-D-galactopyranose-(1-2)-[alpha-D-Abequopyranose-(1-3)]alpha-D-mannopyranose, ... (4 entities in total)
• Functional Keywords: immunoglobulin
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 2
• Total formula weight: 47151.51

Authors

Cygler, M. (deposition date: 1993-10-25, release date: 1994-01-31, Last modification date: 2024-10-30)

Primary citation

Cygler, M.,Rose, D.R.,Bundle, D.R.
Recognition of a cell-surface oligosaccharide of pathogenic Salmonella by an antibody Fab fragment.
Science, 253:442-445, 1991

PubMed Abstract: The 2.05 angstrom (A) resolution crystal structure of a dodecasaccharide-Fab complex revealed an unusual carbohydrate recognition site, defined by aromatic amino acids and a structured water molecule, rather than the carboxylic acid and amide side chains and a structured water molecule, rather than the carboxylic acid and amide side chains that are features of transport and other carbohydrate binding proteins. A trisaccharide epitope of a branched bacterial lipopolysaccharide fills this hydrophobic pocket (8 A deep by 7 A wide) in an entropy-assisted association (association constant = 2.05 x 10(5) liters per mole, enthalpy = -20.5 +/- 1.7 kilojoules per mole, and temperature times entropy = +10.0 +/- 2.9 kilojoules per mole). The requirement for the complementarity of van der Waals surfaces and the requirements of saccharide-saccharide and protein-saccharide hydrogen-bonding networks determine the antigen conformation adopted in the bound state.

PubMed: 1713710

Experimental method

X-RAY DIFFRACTION (2 Å)