1MFE

RECOGNITION OF A CELL-SURFACE OLIGO-SACCHARIDE OF PATHOGENIC SALMONELLA BY AN ANTIBODY FAB FRAGMENT

1MFE の概要

• エントリーDOI: 10.2210/pdb1mfe/pdb
• 分子名称: IGG1-LAMBDA SE155-4 FAB (LIGHT CHAIN), IGG1-LAMBDA SE155-4 FAB (HEAVY CHAIN), alpha-D-galactopyranose-(1-2)-[alpha-D-Abequopyranose-(1-3)]alpha-D-mannopyranose, ... (4 entities in total)
• 機能のキーワード: immunoglobulin
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47151.51

構造登録者

Cygler, M. (登録日: 1993-10-25, 公開日: 1994-01-31, 最終更新日: 2024-10-30)

主引用文献

Cygler, M.,Rose, D.R.,Bundle, D.R.
Recognition of a cell-surface oligosaccharide of pathogenic Salmonella by an antibody Fab fragment.
Science, 253:442-445, 1991

PubMed Abstract: The 2.05 angstrom (A) resolution crystal structure of a dodecasaccharide-Fab complex revealed an unusual carbohydrate recognition site, defined by aromatic amino acids and a structured water molecule, rather than the carboxylic acid and amide side chains and a structured water molecule, rather than the carboxylic acid and amide side chains that are features of transport and other carbohydrate binding proteins. A trisaccharide epitope of a branched bacterial lipopolysaccharide fills this hydrophobic pocket (8 A deep by 7 A wide) in an entropy-assisted association (association constant = 2.05 x 10(5) liters per mole, enthalpy = -20.5 +/- 1.7 kilojoules per mole, and temperature times entropy = +10.0 +/- 2.9 kilojoules per mole). The requirement for the complementarity of van der Waals surfaces and the requirements of saccharide-saccharide and protein-saccharide hydrogen-bonding networks determine the antigen conformation adopted in the bound state.

PubMed: 1713710

実験手法

X-RAY DIFFRACTION (2 Å)