1MF7

INTEGRIN ALPHA M I DOMAIN

1MF7 の概要

• エントリーDOI: 10.2210/pdb1mf7/pdb
• 分子名称: INTEGRIN ALPHA M (2 entities in total)
• 機能のキーワード: cell adhesion
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P11215
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22226.42

構造登録者

McCleverty, C.J.,Liddington, R.C. (登録日: 2002-08-09, 公開日: 2003-05-20, 最終更新日: 2024-10-09)

主引用文献

McCleverty, C.J.,Liddington, R.C.
Engineered allosteric mutants of the integrin alphaMbeta2 I domain: structural and functional studies
Biochem.J., 372:121-127, 2003

PubMed Abstract: The alpha-I domain, found in the alpha-subunit of the leucocyte integrins such as alphaMbeta2 and alphaLbeta2, switches between the open and closed tertiary conformations, reflecting the high- and low-affinity ligand-binding states of the integrin that are required for regulated cell adhesion and migration. In the present study we show, by using point mutations and engineered disulphide bonds, that ligand affinity can be reduced or increased allosterically by altering the equilibrium between the closed and open states. We determined equilibrium constants for the binding of two ligands, fibrinogen and intercellular cell-adhesion molecule 1, to the alphaM-I domain by surface plasmon resonance, and determined crystal structures of a low-affinity mutant. Locking the domain in the open conformation increases affinity by a factor of no greater than 10, consistent with a closely balanced equilibrium between the two conformations in the absence of ligand. This behaviour contrasts with that of the unliganded alphaL-I domain, for which the equilibrium lies strongly in favour of the closed conformation. These results suggest significant differences in the way the parent integrins regulate I domain conformation and hence ligand affinity.

PubMed: 12611591
DOI: 10.1042/BJ20021273

実験手法

X-RAY DIFFRACTION (1.25 Å)