1MEY

CRYSTAL STRUCTURE OF A DESIGNED ZINC FINGER PROTEIN BOUND TO DNA

1MEY の概要

• エントリーDOI: 10.2210/pdb1mey/pdb
• 分子名称: DNA (5'-D(*AP*TP*GP*AP*GP*GP*CP*AP*GP*AP*AP*CP*T)-3'), DNA (5'-D(*TP*AP*GP*TP*TP*CP*TP*GP*CP*CP*TP*(C38)P*A)-3'), CONSENSUS ZINC FINGER, ... (6 entities in total)
• 機能のキーワード: zinc finger, protein-dna interaction, protein design, complex (zinc finger-dna), transferase-dna complex, transferase/dna
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 47137.17

構造登録者

Kim, C.A.,Berg, J.M. (登録日: 1996-09-27, 公開日: 1997-03-12, 最終更新日: 2024-02-14)

主引用文献

Kim, C.A.,Berg, J.M.
A 2.2 A Resolution Crystal Structure of a Designed Zinc Finger Protein Bound to DNA
Nat.Struct.Biol., 3:940-945, 1996

PubMed Abstract: Considerable recent effort has been devoted to the design and selection of sequence-specific DNA binding proteins based on tandem arrays of Cys2His2 zinc finger domains. While the DNA binding properties of these designed proteins have been studied extensively, the structural basis for site-specific binding has not been examined experimentally. Here we report the crystal structure of a complex between a protein comprised of three consensus-sequence-based zinc finger domains and an oligonucleotide corresponding to a favourable DNA binding site. This structure reveals relatively simple modular interactions and structural adaptations that compensate for differences in contact residue side-chain lengths.

PubMed: 8901872
DOI: 10.1038/nsb1196-940

実験手法

X-RAY DIFFRACTION (2.2 Å)