1MEG

CRYSTAL STRUCTURE OF A CARICAIN D158E MUTANT IN COMPLEX WITH E-64

Summary for 1MEG

Entry DOI	10.2210/pdb1meg/pdb
Descriptor	CARICAIN, N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE, ETHANOL, ... (4 entities in total)
Functional Keywords	cysteine proteinase, thiol protease, hydrolase
Biological source	Carica papaya (papaya)
Total number of polymer chains	1
Total formula weight	23746.27
Authors	Katerelos, N.A. (deposition date: 1996-05-04, release date: 1997-01-11, Last modification date: 2024-11-20)
Primary citation	Katerelos, N.A.,Taylor, M.A.,Scott, M.,Goodenough, P.W.,Pickersgill, R.W. Crystal structure of a caricain D158E mutant in complex with E-64. FEBS Lett., 392:35-39, 1996 Cited by PubMed Abstract: The structure of the D158E mutant of caricain (previously known as papaya protease omega) in complex with E-64 has been determined at 2.0 A resolution (overall R factor 19.3%). The structure reveals that the substituted glutamate makes the same pattern of hydrogen bonds as the aspartate in native caricain. This was not anticipated since in the native structure there is insufficient room to accommodate the glutamate side chain. The glutamate is accommodated in the mutant by a local expansion of the structure demonstrating that small structural changes are responsible for the change in activity. PubMed: 8769310 DOI: 10.1016/0014-5793(96)00697-7 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2 Å)

Structure validation