1MEG

CRYSTAL STRUCTURE OF A CARICAIN D158E MUTANT IN COMPLEX WITH E-64

1MEG の概要

• エントリーDOI: 10.2210/pdb1meg/pdb
• 分子名称: CARICAIN, N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE, ETHANOL, ... (4 entities in total)
• 機能のキーワード: cysteine proteinase, thiol protease, hydrolase
• 由来する生物種: Carica papaya (papaya)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23746.27

構造登録者

Katerelos, N.A. (登録日: 1996-05-04, 公開日: 1997-01-11, 最終更新日: 2024-11-20)

主引用文献

Katerelos, N.A.,Taylor, M.A.,Scott, M.,Goodenough, P.W.,Pickersgill, R.W.
Crystal structure of a caricain D158E mutant in complex with E-64.
FEBS Lett., 392:35-39, 1996

PubMed Abstract: The structure of the D158E mutant of caricain (previously known as papaya protease omega) in complex with E-64 has been determined at 2.0 A resolution (overall R factor 19.3%). The structure reveals that the substituted glutamate makes the same pattern of hydrogen bonds as the aspartate in native caricain. This was not anticipated since in the native structure there is insufficient room to accommodate the glutamate side chain. The glutamate is accommodated in the mutant by a local expansion of the structure demonstrating that small structural changes are responsible for the change in activity.

PubMed: 8769310
DOI: 10.1016/0014-5793(96)00697-7

実験手法

X-RAY DIFFRACTION (2 Å)