1MB8

Crystal Structure of the actin binding domain of plectin

1MB8 の概要

• エントリーDOI: 10.2210/pdb1mb8/pdb
• 分子名称: Plectin (2 entities in total)
• 機能のキーワード: calponin homology domain, actin binding domain, integrin beta4 hemidesmosomes, cytoskeleton, epidermolysis bullosa, structural protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm, cytoskeleton: Q15149
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28444.57

構造登録者

de Pereda, J.M. (登録日: 2002-08-02, 公開日: 2003-06-10, 最終更新日: 2024-02-14)

主引用文献

Garcia-Alvarez, B.,Bobkov, A.,Sonnenberg, A.,de Pereda, J.M.
Structural and Functional Analysis of the Actin Binding Domain of Plectin Suggests Alternative Mechanisms for Binding to F-Actin and Integrin Beta4
Structure, 11:615-625, 2003

PubMed Abstract: Plectin is a widely expressed cytoskeletal linker. Here we report the crystal structure of the actin binding domain of plectin and show that this region is sufficient for interaction with F-actin or the cytoplasmic region of integrin alpha6beta4. The structure is formed by two calponin homology domains arranged in a closed conformation. We show that binding to F-actin induces a conformational change in plectin that is inhibited by an engineered interdomain disulfide bridge. A two-step induced fit mechanism involving binding and subsequent domain rearrangement is proposed. In contrast, interaction with integrin alpha6beta4 occurs in a closed conformation. Competitive binding of plectin to F-actin and integrin alpha6beta4 may rely on the observed alternative binding mechanisms and involve both allosteric and steric factors.

PubMed: 12791251
DOI: 10.1016/S0969-2126(03)00090-X

実験手法

X-RAY DIFFRACTION (2.15 Å)