1M4M

Mouse Survivin

Summary for 1M4M

• Entry DOI: 10.2210/pdb1m4m/pdb
• Descriptor: BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 5, ZINC ION (2 entities in total)
• Functional Keywords: zn finger baculovirus iap repeat, apoptosis
• Biological source: Mus musculus (house mouse)
• Cellular location: Cytoplasm (By similarity): O70201
• Total number of polymer chains: 1
• Total formula weight: 16450.37

Authors

Muchmore, S.W.,Chen, J.,Jakob, C.,Zakula, D.,Matayoshi, E.D.,Wu, W.,Zhang, H.,Li, F.,Ng, S.C.,Altieri, D.C. (deposition date: 2002-07-03, release date: 2002-09-25, Last modification date: 2024-10-30)

Primary citation

Muchmore, S.W.,Chen, J.,Jakob, C.,Zakula, D.,Matayoshi, E.D.,Wu, W.,Zhang, H.,Li, F.,Ng, S.C.,Altieri, D.C.
CRYSTAL STRUCTURE AND MUTAGENIC ANALYSIS OF THE INHIBITOR-OF-APOPTOSIS PROTEIN SURVIVIN
MOL.CELL, 6:173-182, 2000

PubMed Abstract: The coupling of apoptosis (programmed cell death) to the cell division cycle is essential for homeostasis and genomic integrity. Here, we report the crystal structure of survivin, an inhibitor of apoptosis, which has been implicated in both control of cell death and regulation of cell division. In addition to a conserved N-terminal Zn finger baculovirus IAP repeat, survivin forms a dimer through a symmetric interaction with an intermolecularly bound Zn atom located along the molecular dyad axis. The interaction of the dimer-related C-terminal alpha helices forms an extended surface of approximately 70 A in length. Mutagenesis analysis revealed that survivin dimerization and an extended negatively charged surface surrounding Asp-71 are required to counteract apoptosis and preserve ploidy. These findings may provide a structural basis for a dual role of survivin in inhibition of apoptosis and regulation of cell division.

PubMed: 10949038
DOI: 10.1016/S1097-2765(00)00018-6

Experimental method

X-RAY DIFFRACTION (2.8 Å)