1LVO

Structure of coronavirus main proteinase reveals combination of a chymotrypsin fold with an extra alpha-helical domain

1LVO の概要

• エントリーDOI: 10.2210/pdb1lvo/pdb
• 分子名称: Replicase, hydrolase domain, SULFATE ION, 1,4-DIETHYLENE DIOXIDE, ... (5 entities in total)
• 機能のキーワード: 3c like, corona, proteinase, chymotrypsin, cysteine histidine dyad, beta barrel, hydrolase
• 由来する生物種: Transmissible gastroenteritis virus
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 202836.08

構造登録者

Anand, K.,Palm, G.J.,Mesters, J.R.,Siddell, S.G.,Ziebuhr, J.,Hilgenfeld, R. (登録日: 2002-05-29, 公開日: 2002-07-17, 最終更新日: 2024-03-13)

主引用文献

Anand, K.,Palm, G.J.,Mesters, J.R.,Siddell, S.G.,Ziebuhr, J.,Hilgenfeld, R.
Structure of coronavirus main proteinase reveals combination of a chymotrypsin fold with an extra alpha-helical domain.
EMBO J., 21:3213-3224, 2002

PubMed Abstract: The key enzyme in coronavirus polyprotein processing is the viral main proteinase, M(pro), a protein with extremely low sequence similarity to other viral and cellular proteinases. Here, the crystal structure of the 33.1 kDa transmissible gastroenteritis (corona)virus M(pro) is reported. The structure was refined to 1.96 A resolution and revealed three dimers in the asymmetric unit. The mutual arrangement of the protomers in each of the dimers suggests that M(pro) self-processing occurs in trans. The active site, comprised of Cys144 and His41, is part of a chymotrypsin-like fold that is connected by a 16 residue loop to an extra domain featuring a novel alpha-helical fold. Molecular modelling and mutagenesis data implicate the loop in substrate binding and elucidate S1 and S2 subsites suitable to accommodate the side chains of the P1 glutamine and P2 leucine residues of M(pro) substrates. Interactions involving the N-terminus and the alpha-helical domain stabilize the loop in the orientation required for trans-cleavage activity. The study illustrates that RNA viruses have evolved unprecedented variations of the classical chymotrypsin fold.

PubMed: 12093723
DOI: 10.1093/emboj/cdf327

実験手法

X-RAY DIFFRACTION (1.96 Å)