1LV9

CXCR3 Binding Chemokine IP-10/CXCL10

1LV9 の概要

• エントリーDOI: 10.2210/pdb1lv9/pdb
• NMR情報: BMRB: 5383
• 分子名称: Small inducible cytokine B10 (1 entity in total)
• 機能のキーワード: chemokine, cytokine
• 細胞内の位置: Secreted: P02778
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8651.37

構造登録者

Booth, V.,Keizer, D.W.,Kamphuis, M.B.,Clark-Lewis, I.,Sykes, B.D. (登録日: 2002-05-27, 公開日: 2002-09-18, 最終更新日: 2024-10-30)

主引用文献

Booth, V.,Keizer, D.W.,Kamphuis, M.B.,Clark-Lewis, I.,Sykes, B.D.
The CXCR3 binding chemokine IP-10/CXCL10: structure and receptor interactions.
Biochemistry, 41:10418-10425, 2002

PubMed Abstract: The structure of IP-10 was solved by NMR spectroscopy and represents the first structure from the class of agonists toward the receptor CXCR3. CXCR3 binding chemokines are unique in their ability to bind receptors from both the CC and CXC classes of chemokine receptors. An unusual structural feature of IP-10 was identified that may provide the basis for the ability of IP-10 to bind both CXCR3 and CCR3. The surface of IP-10 that interacts with the N-terminus of CXCR3 was defined by monitoring changes in the NMR spectrum of IP-10 upon addition of a CXCR3 N-terminal peptide. These studies indicated that the interaction involves a hydrophobic cleft, formed by the N-loop and 40s-loop region of IP-10, similar to the interaction surface observed for other chemokines such as IL-8. An additional region of interaction was observed that consists of a hydrophobic cleft formed by the N-terminus of IP-10 and 30s-loop of IP-10.

PubMed: 12173928
DOI: 10.1021/bi026020q

実験手法

SOLUTION NMR