1LV2

Hepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty Acids

1LV2 の概要

• エントリーDOI: 10.2210/pdb1lv2/pdb
• 分子名称: Hepatocyte nuclear factor 4-gamma, PALMITIC ACID (3 entities in total)
• 機能のキーワード: diabetes, fatty acids, hnf4, mody, nuclear receptor, transcription factor, transcription
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus : Q14541
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26111.35

構造登録者

Wisely, B.,Miller, A.B.,Davis, R.G.,Spitzer, T.,Shearer, B.,Moore, J.T.,Johnson, R.,Sefler, A.,Willson, T.M.,Williams, S.P. (登録日: 2002-05-24, 公開日: 2002-12-18, 最終更新日: 2024-04-03)

主引用文献

Wisely, G.B.,Miller, A.B.,Davis, R.G.,Jr Thornquest, A.D.,Johnson, R.,Spitzer, T.,Sefler, A.,Shearer, B.,Moore, J.T.,Willson, T.M.,Williams, S.P.
Hepatocyte Nuclear Factor 4 Is a Transcription Factor that Constitutively Binds Fatty Acids.
Structure, 10:1225-1234, 2002

PubMed Abstract: The 2.7 A X-ray crystal structure of the HNF4gamma ligand binding domain (LBD) revealed the presence of a fatty acid within the pocket, with the AF2 helix in a conformation characteristic of a transcriptionally active nuclear receptor. GC/MS and NMR analysis of chloroform/methanol extracts from purified HNF4alpha and HNF4gamma LBDs identified mixtures of saturated and cis-monounsaturated C14-18 fatty acids. The purified HNF4 LBDs interacted with nuclear receptor coactivators, and both HNF4 subtypes show high constitutive activity in transient transfection assays, which was reduced by mutations designed to interfere with fatty acid binding. The endogenous fatty acids did not readily exchange with radiolabeled palmitic acid, and all attempts to displace them without denaturing the protein failed. Our results suggest that the HNF4s may be transcription factors that are constitutively bound to fatty acids.

PubMed: 12220494
DOI: 10.1016/S0969-2126(02)00829-8

実験手法

X-RAY DIFFRACTION (2.7 Å)