1LUC

BACTERIAL LUCIFERASE

1LUC の概要

• エントリーDOI: 10.2210/pdb1luc/pdb
• 分子名称: BACTERIAL LUCIFERASE, MAGNESIUM ION, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: monooxygenase, flavoprotein
• 由来する生物種: Vibrio harveyi; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 76965.00

構造登録者

Fisher, A.J.,Rayment, I. (登録日: 1996-05-10, 公開日: 1996-12-07, 最終更新日: 2024-02-14)

主引用文献

Fisher, A.J.,Thompson, T.B.,Thoden, J.B.,Baldwin, T.O.,Rayment, I.
The 1.5-A resolution crystal structure of bacterial luciferase in low salt conditions.
J.Biol.Chem., 271:21956-21968, 1996

PubMed Abstract: Bacterial luciferase is a flavin monooxygenase that catalyzes the oxidation of a long-chain aldehyde and releases energy in the form of visible light. A new crystal form of luciferase cloned from Vibrio harveyi has been grown under low-salt concentrations, which diffract x-rays beyond 1.5-A resolution. The x-ray structure of bacterial luciferase has been refined to a conventional R-factor of 18.2% for all recorded synchrotron data between 30.0 and 1.50-A resolution. Bacterial luciferase is an alpha-beta heterodimer, and the individual subunits fold into a single domain (beta/alpha)8 barrel. The high resolution structure reveals a non-prolyl cis peptide bond that forms between Ala74 and Ala75 in the alpha subunit near the putative active site. This cis peptide bond may have functional significance for creating a cavity at the active site. Bacterial luciferase employs reduced flavin as a substrate rather than a cofactor. The structure presented was determined in the absence of substrates. A comparison of the structural similarities between luciferase and a nonfluorescent flavoprotein, which is expressed in the lux operon of one genus of bioluminescent bacteria, suggests that the two proteins originated from a common ancestor. However, the flavin binding sites of the nonfluorescent protein are likely not representative of the flavin binding site on luciferase. The structure presented here will furnish a detailed molecular model for all bacterial luciferases.

PubMed: 8703001
DOI: 10.1074/jbc.271.36.21956

実験手法

X-RAY DIFFRACTION (1.5 Å)