1LTI

HEAT-LABILE ENTEROTOXIN (LT-I) COMPLEX WITH T-ANTIGEN

1LTI の概要

• エントリーDOI: 10.2210/pdb1lti/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900084
• 分子名称: HEAT LABILE ENTEROTOXIN TYPE I, beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-alpha-D-galactopyranose, beta-D-galactopyranose, ... (6 entities in total)
• 機能のキーワード: adp-ribosyl transferase, enterotoxin
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 87831.12

構造登録者

Van Den Akker, F.,Hol, W.G.J. (登録日: 1996-05-09, 公開日: 1996-08-17, 最終更新日: 2024-11-20)

主引用文献

van den Akker, F.,Steensma, E.,Hol, W.G.
Tumor marker disaccharide D-Gal-beta 1, 3-GalNAc complexed to heat-labile enterotoxin from Escherichia coli.
Protein Sci., 5:1184-1188, 1996

PubMed Abstract: Heat-labile enterotoxin (LT) is part of the cholera toxin (CT) family and consists of a catalytic A subunit and a B pentamer that serves to recognize the oligosaccharide part of the GM1 ganglioside receptor. We report here the crystal structure of heat-labile enterotoxin in complex with the disaccharide portion of the Thomsen-Friedenreich (T-antigen) tumor marker. The toxin:carbohydrate complex is determined to 2.13 A resolution, yielding an R-factor of 18.5%. The T-antigen disaccharide, D-Gal-beta 1,3-GalNAc-Ser/Thr, is present in more than 85% of human carcinomas and monitoring its autoimmune response is used for the early detection of tumors. Insight into the molecular recognition of this tumor antigen by sugar binding proteins can benefit the development of a diagnostic tool for human carcinomas as well as a T-antigen directed anticancer drug delivery system.

PubMed: 8762150

実験手法

X-RAY DIFFRACTION (2.13 Å)