1LSG

THREE-DIMENSIONAL STRUCTURE OF THE PLATELET INTEGRIN RECOGNITION SEGMENT OF THE FIBRINOGEN GAMMA CHAIN OBTAINED BY CARRIER PROTEIN-DRIVEN CRYSTALLIZATION

1LSG の概要

• エントリーDOI: 10.2210/pdb1lsg/pdb
• 分子名称: HEN EGG WHITE LYSOZYME (1 entity in total)
• 機能のキーワード: lysozyme, fibrinogen, hybrid protein
• 由来する生物種: Gallus gallus (chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15892.90

構造登録者

Patel, H.,Anderson, W.F. (登録日: 1994-10-25, 公開日: 1995-09-15, 最終更新日: 2024-11-20)

主引用文献

Donahue, J.P.,Patel, H.,Anderson, W.F.,Hawiger, J.
Three-dimensional structure of the platelet integrin recognition segment of the fibrinogen gamma chain obtained by carrier protein-driven crystallization.
Proc.Natl.Acad.Sci.USA, 91:12178-12182, 1994

PubMed Abstract: We have developed a method for crystallizing small functional protein segments so that their three-dimensional structure can be determined by x-ray diffraction analysis. This method consists of linking a small protein segment of unknown tertiary structure to either the amino or carboxyl terminus of a larger carrier protein of known tertiary structure. Crystallization of the small segment is then driven by crystallization of the carrier protein. Using this approach, we have obtained crystals of the human fibrinogen gamma-chain carboxyl-terminal segment linked to the carboxyl terminus of chicken egg white lysozyme. The three-dimensional structure of the carboxyl-terminal segment of the fibrinogen gamma chain was determined by x-ray diffraction analysis at a resolution of 2.4 A. This segment encompasses the recognition site for the integrin alpha IIb beta 3 receptor on activated platelets and for the clumping receptor on pathogenic staphylococci and also bears donor and acceptor sites for factor XIIIa-catalyzed crosslinking of fibrin. Therefore, the structural information derived from our analysis will provide a rational basis for the design of inhibitors of these important functions of fibrinogen. Moreover, carrier protein-driven crystallization will facilitate the determination of the three-dimensional structure of functional segments of other proteins that are, like fibrinogen, difficult to crystallize in toto.

PubMed: 7527555
DOI: 10.1073/pnas.91.25.12178

実験手法

X-RAY DIFFRACTION (2.4 Å)