1LRI

BETA-CRYPTOGEIN-CHOLESTEROL COMPLEX

1LRI の概要

• エントリーDOI: 10.2210/pdb1lri/pdb
• 関連するPDBエントリー: 1BEO 1BXM
• 分子名称: Beta-elicitin cryptogein, CHLORIDE ION, CHOLESTEROL, ... (4 entities in total)
• 機能のキーワード: cryptogein, cholesterol, sterol carrier protein, toxin
• 由来する生物種: Phytophthora cryptogea
• 細胞内の位置: Secreted: P15570
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10748.90

構造登録者

Lascombe, M.-B.,Ponchet, M.,Venard, P.,Milat, M.-L.,Blein, J.-P.,Prange, T. (登録日: 2002-05-15, 公開日: 2002-05-29, 最終更新日: 2024-10-30)

主引用文献

Lascombe, M.B.,Ponchet, M.,Venard, P.,Milat, M.L.,Blein, J.P.,Prange, T.
The 1.45 A resolution structure of the cryptogein-cholesterol complex: a close-up view of a sterol carrier protein (SCP) active site.
Acta Crystallogr.,Sect.D, 58:1442-1447, 2002

PubMed Abstract: Cryptogein is a small 10 kDa elicitor produced by the phytoparasitic oomycete Phytophthora cryptogea. The protein also displays a sterol carrier activity. The native protein crystallizes in space group P4(1)22, with unit-cell parameters a = b = 46.51, c = 134.9 A (diffraction limit: 2.1 A). Its complex with cholesterol crystallizes in space group C222(1), with unit-cell parameters a = 30.96, b = 94.8, c = 65.3 A and a resolution enhanced to 1.45 A. The large inner non-specific hydrophobic cavity is able to accommodate a large variety of 3-beta-hydroxy sterols. Cryptogein probably acts as a sterol shuttle helping the pathogen to grow and complete its life cycle.

PubMed: 12198300
DOI: 10.1107/S0907444902011745

実験手法

X-RAY DIFFRACTION (1.45 Å)