1LPV

DROSOPHILA MELANOGASTER DOUBLESEX (DSX), NMR, 18 STRUCTURES

1LPV の概要

• エントリーDOI: 10.2210/pdb1lpv/pdb
• 分子名称: Doublesex protein, ZINC ION (2 entities in total)
• 機能のキーワード: transcription, drosophila melanogaster, dna binding, gene regulation
• 細胞内の位置: Nucleus: P23023
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6256.15

構造登録者

Zhu, L.,Wilken, J.,Phillips, N.,Narendra, U.,Chan, G.,Stratton, S.,Kent, S.,Weiss, M.A. (登録日: 2002-05-08, 公開日: 2002-10-02, 最終更新日: 2024-05-22)

主引用文献

Zhu, L.,Wilken, J.,Phillips, N.B.,Narendra, U.,Chan, G.,Stratton, S.M.,Kent, S.B.,Weiss, M.A.
Sexual dimorphism in diverse metazoans is regulated by a novel class of intertwined zinc fingers.
Genes Dev., 14:1750-1764, 2000

PubMed Abstract: Sex determination is regulated by diverse pathways. Although upstream signals vary, a cysteine-rich DNA-binding domain (the DM motif) is conserved within downstream transcription factors of Drosophila melanogaster (Doublesex) and Caenorhabditis elegans (MAB-3). Vertebrate DM genes have likewise been identified and, remarkably, are associated with human sex reversal (46, XY gonadal dysgenesis). Here we demonstrate that the structure of the Doublesex domain contains a novel zinc module and disordered tail. The module consists of intertwined CCHC and HCCC Zn(2+)-binding sites; the tail functions as a nascent recognition alpha-helix. Mutations in either Zn(2+)-binding site or tail can lead to an intersex phenotype. The motif binds in the DNA minor groove without sharp DNA bending. These molecular features, unusual among zinc fingers and zinc modules, underlie the organization of a Drosophila enhancer that integrates sex- and tissue-specific signals. The structure provides a foundation for analysis of DM mutations affecting sexual dimorphism and courtship behavior.

PubMed: 10898790
DOI: 10.1101/gad.189500

実験手法

SOLUTION NMR