1LO5

Crystal structure of the D227A variant of Staphylococcal enterotoxin A in complex with human MHC class II

1LO5 の概要

• エントリーDOI: 10.2210/pdb1lo5/pdb
• 関連するPDBエントリー: 1dlh 1esf
• 分子名称: HLA class II histocompatibility antigen, DR alpha chain, HLA class II histocompatibility antigen, DR-1 beta chain, Hemagglutinin peptide, ... (5 entities in total)
• 機能のキーワード: protein-protein complex, immune system-toxin complex, immune system/toxin
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cell membrane; Single-pass type I membrane protein: P01903 P04229; Secreted: P0A0L2
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 71829.67

構造登録者

Petersson, K.,Thunnissen, M.,Forsberg, G.,Walse, B. (登録日: 2002-05-06, 公開日: 2002-12-18, 最終更新日: 2024-10-09)

主引用文献

Petersson, K.,Thunnissen, M.,Forsberg, G.,Walse, B.
Crystal Structure of a SEA Variant in Complex with MHC Class II Reveals the Ability of SEA to Crosslink MHC Molecules
Structure, 10:1619-1626, 2002

PubMed Abstract: Although the biological properties of staphylococcal enterotoxin A (SEA) have been well characterized, structural insights into the interaction between SEA and major histocompatibilty complex (MHC) class II have only been obtained by modeling. Here, the crystal structure of the D227A variant of SEA in complex with human MHC class II has been determined by X-ray crystallography. SEA(D227A) exclusively binds with its N-terminal domain to the alpha chain of HLA-DR1. The ability of one SEA molecule to crosslink two MHC molecules was modeled. It shows that this SEA molecule cannot interact with the T cell receptor (TCR) while a second SEA molecule interacts with MHC. Because of its relatively low toxicity, the D227A variant of SEA is used in tumor therapy.

PubMed: 12467569
DOI: 10.1016/S0969-2126(02)00895-X

実験手法

X-RAY DIFFRACTION (3.2 Å)