1LO1

ESTROGEN RELATED RECEPTOR 2 DNA BINDING DOMAIN IN COMPLEX WITH DNA

1LO1 の概要

• エントリーDOI: 10.2210/pdb1lo1/pdb
• NMR情報: BMRB: 5363
• 分子名称: 5'-D(*GP*CP*TP*CP*AP*AP*GP*GP*TP*CP*AP*CP*G)-3', 5'-D(*CP*GP*TP*GP*AP*CP*CP*TP*TP*GP*AP*GP*C)-3', Steroid hormone receptor ERR2, ... (4 entities in total)
• 機能のキーワード: estrogen related receptor 2, dna binding domain, herr2, hormone nuclear receptor, hormone-growth factor receptor-dna complex, hormone/growth factor receptor/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus (Potential): O95718
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 19223.11

構造登録者

Gearhart, M.D.,Holmbeck, S.M.A.,Evans, R.M.,Dyson, H.J.,Wright, P.E. (登録日: 2002-05-05, 公開日: 2003-04-22, 最終更新日: 2024-05-22)

主引用文献

Gearhart, M.D.,Holmbeck, S.M.A.,Evans, R.M.,Dyson, H.J.,Wright, P.E.
Monomeric Complex of Human Orphan Estrogen Related Receptor-2 with DNA: A Pseudo-dimer Interface Mediates Extended Half-site Recognition
J.Mol.Biol., 327:819-832, 2003

PubMed Abstract: While most nuclear receptors bind DNA as homo or heterodimers, the human estrogen related receptors (hERRs) are members of a subfamily of orphan receptors that bind DNA as monomers. We have determined the solution structure of the DNA binding domain (DBD) of hERR2 bound to its cognate DNA. The structure and base interactions of the core DBD are similar to those of other nuclear receptors. However, high-affinity, sequence-specific DNA binding as a monomer necessitates formation of additional base contacts outside the core DBD. This is accomplished using a modified guanosine-binding "AT-hook" within the C-terminal extension (CTE) flanking the DBD, which makes base-specific minor groove interactions. The structure of the CTE is stabilized both by interactions with the DNA and by packing against a region of the core DBD normally reserved for dimerization. This pseudo-dimer interface provides a basis for the expansion of DNA recognition and suggests a mechanism through which dimerization may have evolved from an ancestral monomeric receptor.

PubMed: 12654265
DOI: 10.1016/S0022-2836(03)00183-9

実験手法

SOLUTION NMR