1LJE

CRYSTAL STRUCTURE OF MONOCLINIC LYSOZYME GROWN IN PRESENCE OF 10% SUCROSE

Summary for 1LJE

• Entry DOI: 10.2210/pdb1lje/pdb
• Related: 1LJ3 1LJ4 1LJF 1LJG 1LJH 1LJI 1LJJ 1LJK
• Descriptor: Lysozyme C, NITRATE ION (3 entities in total)
• Functional Keywords: hydration of proteins, hydrolase
• Biological source: Gallus gallus (chicken)
• Cellular location: Secreted: P00698
• Total number of polymer chains: 2
• Total formula weight: 29034.35

Authors

Saraswathi, N.T.,Sankaranarayanan, R.,Vijayan, M. (deposition date: 2002-04-21, release date: 2002-10-19, Last modification date: 2024-11-06)

Primary citation

Saraswathi, N.T.,Sankaranarayanan, R.,Vijayan, M.
Effect of stabilizing additives on the structure and hydration of proteins: a study involving monoclinic lysozyme.
Acta Crystallogr.,Sect.D, 58:1162-1167, 2002

PubMed Abstract: In pursuance of a long-range programme on the hydration, mobility and action of proteins, the structural basis of the stabilizing effect of sugars and polyols is being investigated. With two crystallographically independent molecules with slightly different packing environments in the crystal, monoclinic lysozyme constitutes an ideal system for exploring the problem. The differences in the structure and hydration of the two molecules provide a framework for examining the changes caused by stabilizing additives. Monoclinic crystals were grown under native conditions and also in the presence of 10% sucrose, 15% trehalose, 10% trehalose, 10% sorbitol and 5% glycerol. The crystal structures were refined at resolutions ranging from 1.8 to 2.1 A. The average B values, and hence the mobility of the structure, are lower in the presence of additives than in the native crystals. However, a comparison of the structures indicates that the effect of the additives on the structure and the hydration shell around the protein molecule is considerably less than that caused by differences in packing. It is also less than that caused by the replacement of NaNO(3) by NaCl as the precipitant in the crystallization experiments. This result is not in conformity with the commonly held belief that additives exert their stabilizing effect through the reorganization of the hydration shell, at least as far as the ordered water molecules are concerned.

PubMed: 12077436
DOI: 10.1107/S0907444902007126

Experimental method

X-RAY DIFFRACTION (2 Å)