1LG7

Crystal structure of Vesicular Stomatitis Virus Matrix Protein

1LG7 の概要

• エントリーDOI: 10.2210/pdb1lg7/pdb
• 分子名称: VSV matrix protein (2 entities in total)
• 機能のキーワード: virus matrix, viral protein
• 由来する生物種: Vesicular stomatitis virus
• 細胞内の位置: Virion membrane; Peripheral membrane protein: Q8B0H2
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21011.04

構造登録者

Gaudier, M.,Gaudin, Y.,Knossow, M. (登録日: 2002-04-15, 公開日: 2002-06-19, 最終更新日: 2024-11-13)

主引用文献

Gaudier, M.,Gaudin, Y.,Knossow, M.
Crystal structure of vesicular stomatitis virus matrix protein.
EMBO J., 21:2886-2892, 2002

PubMed Abstract: The vesicular stomatitis virus (VSV) matrix protein (M) interacts with cellular membranes, self-associates and plays a major role in virus assembly and budding. We present the crystallographic structure, determined at 1.96 A resolution, of a soluble thermolysin resistant core of VSV M. The fold is a new fold shared by the other vesiculovirus matrix proteins. The structure accounts for the loss of stability of M temperature-sensitive mutants deficient in budding, and reveals a flexible loop protruding from the globular core that is important for self-assembly. Membrane floatation shows that, together with the M lysine-rich N-terminal peptide, a second domain of the protein is involved in membrane binding. Indeed, the structure reveals a hydrophobic surface located close to the hydrophobic loop and surrounded by conserved basic residues that may constitute this domain. Lastly, comparison of the negative-stranded virus matrix proteins with retrovirus Gag proteins suggests that the flexible link between their major membrane binding domain and the rest of the structure is a common feature shared by these proteins involved in budding and virus assembly.

PubMed: 12065402
DOI: 10.1093/emboj/cdf284

実験手法

X-RAY DIFFRACTION (1.96 Å)