1LE2

STRUCTURAL BASIS FOR ALTERED FUNCTION IN THE COMMON MUTANTS OF HUMAN APOLIPOPROTEIN-E

1LE2 の概要

• エントリーDOI: 10.2210/pdb1le2/pdb
• 分子名称: APOLIPOPROTEIN E2 (1 entity in total)
• 機能のキーワード: lipoprotein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P02649
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16689.03

構造登録者

Wilson, C.,Agard, D.A. (登録日: 1991-08-22, 公開日: 1992-10-15, 最終更新日: 2024-02-14)

主引用文献

Wilson, C.,Mau, T.,Weisgraber, K.H.,Wardell, M.R.,Mahley, R.W.,Agard, D.A.
Salt bridge relay triggers defective LDL receptor binding by a mutant apolipoprotein.
Structure, 2:713-718, 1994

PubMed Abstract: Apolipoprotein-E (apo-E), a 34kDa blood plasma protein, plays a key role in directing cholesterol transport via its interaction with the low density lipoprotein (LDL) receptor. The amino-terminal domain of apo-E forms an unusually elongated four-helix bundle arranged such that key basic residues involved in LDL receptor binding form a cluster at the end of one of the helices. A common apo-E variant, apo-E2, corresponding to the single-site substitution Arg158-->Cys, displays minimal LDL receptor binding and is associated with significant changes in plasma cholesterol levels and increased risk of coronary heart disease. Surprisingly, the site of mutation in this variant is physically well removed (> 12A) from the cluster of LDL receptor binding residues.

PubMed: 7994571
DOI: 10.1016/S0969-2126(00)00072-1

実験手法

X-RAY DIFFRACTION (3 Å)