1LC4
Crystal Structure of Tobramycin Bound to the Eubacterial 16S rRNA A Site
Summary for 1LC4
| Entry DOI | 10.2210/pdb1lc4/pdb |
| Related | 1J7T |
| Descriptor | 5'-R(*UP*UP*GP*CP*GP*UP*CP*AP*CP*AP*CP*CP*GP*GP*UP*GP*AP*AP*GP*UP*CP*GP*C)-3', TOBRAMYCIN (3 entities in total) |
| Functional Keywords | antibiotic-rna complex, adenine bulges, rna |
| Total number of polymer chains | 2 |
| Total formula weight | 15645.85 |
| Authors | Vicens, Q.,Westhof, E. (deposition date: 2002-04-05, release date: 2003-04-08, Last modification date: 2023-08-16) |
| Primary citation | Vicens, Q.,Westhof, E. Crystal Structure of a Complex between the Aminoglycoside Tobramycin and an Oligonucleotide Containing the Ribosomal Decoding A Site Chem.Biol., 9:747-755, 2002 Cited by PubMed Abstract: Aminoglycoside antibiotics target the decoding aminoacyl site (A site) on the 16S ribosomal RNA and induce miscoding during translation. Here, we present the crystal structure, at 2.54 A resolution, of an RNA oligonucleotide containing the A site sequence complexed to the 4,6-disubstituted 2-deoxystreptamine aminoglycoside tobramycin. The three aminosugar rings making up tobramycin interact with the deep-groove atoms directly or via water molecules and stabilize a fully bulged-out conformation of adenines A(1492) and A(1493). The comparison between this structure and the one previously solved in the presence of paromomycin confirms the importance of the functional groups on the common neamine part of these two antibiotics for binding to RNA. Furthermore, the analysis of the present structure provides a molecular explanation to some of the resistance mechanisms that have spread among bacteria and rendered aminoglycoside antibiotics inefficient. PubMed: 12079787DOI: 10.1016/S1074-5521(02)00153-9 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.54 Å) |
Structure validation
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