1LC4

Crystal Structure of Tobramycin Bound to the Eubacterial 16S rRNA A Site

1LC4 の概要

• エントリーDOI: 10.2210/pdb1lc4/pdb
• 関連するPDBエントリー: 1J7T
• 分子名称: 5'-R(*UP*UP*GP*CP*GP*UP*CP*AP*CP*AP*CP*CP*GP*GP*UP*GP*AP*AP*GP*UP*CP*GP*C)-3', TOBRAMYCIN (3 entities in total)
• 機能のキーワード: antibiotic-rna complex, adenine bulges, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15645.85

構造登録者

Vicens, Q.,Westhof, E. (登録日: 2002-04-05, 公開日: 2003-04-08, 最終更新日: 2023-08-16)

主引用文献

Vicens, Q.,Westhof, E.
Crystal Structure of a Complex between the Aminoglycoside Tobramycin and an Oligonucleotide Containing the Ribosomal Decoding A Site
Chem.Biol., 9:747-755, 2002

PubMed Abstract: Aminoglycoside antibiotics target the decoding aminoacyl site (A site) on the 16S ribosomal RNA and induce miscoding during translation. Here, we present the crystal structure, at 2.54 A resolution, of an RNA oligonucleotide containing the A site sequence complexed to the 4,6-disubstituted 2-deoxystreptamine aminoglycoside tobramycin. The three aminosugar rings making up tobramycin interact with the deep-groove atoms directly or via water molecules and stabilize a fully bulged-out conformation of adenines A(1492) and A(1493). The comparison between this structure and the one previously solved in the presence of paromomycin confirms the importance of the functional groups on the common neamine part of these two antibiotics for binding to RNA. Furthermore, the analysis of the present structure provides a molecular explanation to some of the resistance mechanisms that have spread among bacteria and rendered aminoglycoside antibiotics inefficient.

PubMed: 12079787
DOI: 10.1016/S1074-5521(02)00153-9

実験手法

X-RAY DIFFRACTION (2.54 Å)