1L3A
Structure of the plant transcriptional regulator PBF-2
Summary for 1L3A
| Entry DOI | 10.2210/pdb1l3a/pdb |
| Descriptor | p24: plant transcriptional regulator PBF-2 (2 entities in total) |
| Functional Keywords | pbf-2, single strand dna binding protein, plant, whirly, transcriptionnal activator, cyclic c4 symmetry, dna binding protein |
| Biological source | Solanum tuberosum (potato) |
| Total number of polymer chains | 4 |
| Total formula weight | 101653.80 |
| Authors | Desveaux, D.,Allard, J.,Brisson, N.,Sygusch, J. (deposition date: 2002-02-26, release date: 2002-06-26, Last modification date: 2024-02-14) |
| Primary citation | Desveaux, D.,Allard, J.,Brisson, N.,Sygusch, J. A new family of plant transcription factors displays a novel ssDNA-binding surface. Nat.Struct.Biol., 9:512-517, 2002 Cited by PubMed Abstract: The crystal structure of p24, the single-stranded DNA (ssDNA) binding subunit of the plant defense transcription factor PBF-2, has been determined to 2.3 A resolution. p24 is representative of a novel family of ubiquitous plant-specific proteins that we refer to as the Whirly family because of their quaternary structure. PBF-2 is composed of four p24 molecules that interact through a helix-loop-helix motif. This interaction produces a central pore, with beta-strands radiating outwards, resulting in a whirligig appearance to the quaternary structure. The noncrystallographic C(4) symmetry arrangement of p24 subunits is novel for ssDNA binding proteins and may explain the binding specificity of PBF-2. This structural arrangement also supports the role of PBF-2 in binding melted promoter regions to modulate gene expression. PubMed: 12080340DOI: 10.1038/nsb814 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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