1L3A

Structure of the plant transcriptional regulator PBF-2

1L3A の概要

• エントリーDOI: 10.2210/pdb1l3a/pdb
• 分子名称: p24: plant transcriptional regulator PBF-2 (2 entities in total)
• 機能のキーワード: pbf-2, single strand dna binding protein, plant, whirly, transcriptionnal activator, cyclic c4 symmetry, dna binding protein
• 由来する生物種: Solanum tuberosum (potato)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 101653.80

構造登録者

Desveaux, D.,Allard, J.,Brisson, N.,Sygusch, J. (登録日: 2002-02-26, 公開日: 2002-06-26, 最終更新日: 2024-02-14)

主引用文献

Desveaux, D.,Allard, J.,Brisson, N.,Sygusch, J.
A new family of plant transcription factors displays a novel ssDNA-binding surface.
Nat.Struct.Biol., 9:512-517, 2002

PubMed Abstract: The crystal structure of p24, the single-stranded DNA (ssDNA) binding subunit of the plant defense transcription factor PBF-2, has been determined to 2.3 A resolution. p24 is representative of a novel family of ubiquitous plant-specific proteins that we refer to as the Whirly family because of their quaternary structure. PBF-2 is composed of four p24 molecules that interact through a helix-loop-helix motif. This interaction produces a central pore, with beta-strands radiating outwards, resulting in a whirligig appearance to the quaternary structure. The noncrystallographic C(4) symmetry arrangement of p24 subunits is novel for ssDNA binding proteins and may explain the binding specificity of PBF-2. This structural arrangement also supports the role of PBF-2 in binding melted promoter regions to modulate gene expression.

PubMed: 12080340
DOI: 10.1038/nsb814

実験手法

X-RAY DIFFRACTION (2.3 Å)