1L2F

Crystal structure of NusA from Thermotoga maritima: a structure-based role of the N-terminal domain

Summary for 1L2F

• Entry DOI: 10.2210/pdb1l2f/pdb
• Descriptor: N utilization substance protein A (2 entities in total)
• Functional Keywords: nusa, ob fold kh domain, rna polymerase, structural genomics, bsgc structure funded by nih, protein structure initiative, psi, berkeley structural genomics center, transcription
• Biological source: Thermotoga maritima
• Total number of polymer chains: 1
• Total formula weight: 41146.05

Authors

Shin, D.H.,Nguyen, H.H.,Jancarik, J.,Yokota, H.,Kim, R.,Kim, S.H.,Berkeley Structural Genomics Center (BSGC) (deposition date: 2002-02-20, release date: 2003-09-23, Last modification date: 2024-10-30)

Primary citation

Shin, D.H.,Nguyen, H.H.,Jancarik, J.,Yokota, H.,Kim, R.,Kim, S.H.
Crystal structure of NusA from Thermotoga maritima and functional implication of the N-terminal domain.
Biochemistry, 42:13429-13437, 2003

PubMed Abstract: We report the crystal structure of N-utilizing substance A protein (NusA) from Thermotoga maritima (TmNusA), a protein involved in transcriptional pausing, termination, and antitermination. TmNusA has an elongated rod-shaped structure consisting of an N-terminal domain (NTD, residues 1-132) and three RNA binding domains (RBD). The NTD consists of two subdomains, the globular head and the helical body domains, that comprise a unique three-dimensional structure that may be important for interacting with RNA polymerase. The globular head domain possesses a high content of negatively charged residues that may interact with the positively charged flaplike domain of RNA polymerase. The helical body domain is composed of a three-helix bundle that forms a hydrophobic core with the aid of two neighboring beta-strands. This domain shows structural similarity with one of the helical domains of sigma(70) factor from Escherichia coli. One side of the molecular surface shows positive electrostatic potential suitable for nonspecific RNA interaction. The RBD is composed of one S1 domain and two K-homology (KH) domains forming an elongated RNA binding surface. Structural comparison between TmNusA and Mycobacterium tuberculosis NusA reveals a possible hinge motion between NTD and RBD. In addition, a functional implication of the NTD in its interaction with RNA polymerase is discussed.

PubMed: 14621988
DOI: 10.1021/bi035118h

Experimental method

X-RAY DIFFRACTION (2.5 Å)