1L0Z

THE STRUCTURE OF PORCINE PANCREATIC ELASTASE COMPLEXED WITH XENON AND BROMIDE, CRYOPROTECTED WITH DRY PARAFFIN OIL

Summary for 1L0Z

• Entry DOI: 10.2210/pdb1l0z/pdb
• Descriptor: ELASTASE 1, SODIUM ION, BROMIDE ION, ... (5 entities in total)
• Functional Keywords: xenon, bromide, ppe, hydrolase
• Biological source: Sus scrofa (pig)
• Cellular location: Secreted: P00772
• Total number of polymer chains: 1
• Total formula weight: 28640.23

Authors

Tucker, P.A.,Panjikar, S. (deposition date: 2002-02-14, release date: 2002-08-28, Last modification date: 2024-10-30)

Primary citation

Panjikar, S.,Tucker, P.A.
Xenon derivatization of halide-soaked protein crystals.
Acta Crystallogr.,Sect.D, 58:1413-1420, 2002

PubMed Abstract: Crystals of porcine pancreatic elastase can be derived by soaking in high-molarity bromide solutions. These crystals, either in a glycerol-based cryoprotectant solution or in paraffin oil, can be subsequently pressurized under a xenon atmosphere to incorporate xenon. When paraffin oil is used, a larger number of bromide ions are observed on the protein surface. Intensity data collected to lower energy than the bromine absorption edge can be used to determine the xenon position and the resultant phase information can be used to determine the bromine substructure from data collected to higher energy than the bromine absorption edge. The method would appear to have general applicability where large substructures need to be determined.

PubMed: 12198297
DOI: 10.1107/S0907444902011010

Experimental method

X-RAY DIFFRACTION (1.5 Å)