1L0P

CRYSTAL STRUCTURE ANALYSIS OF THE COMPLEX BETWEEN PSYCHROPHILIC ALPHA AMYLASE FROM PSEUDOALTEROMONAS HALOPLANCTIS AND NITRATE

1L0P の概要

• エントリーDOI: 10.2210/pdb1l0p/pdb
• 関連するPDBエントリー: 1AQH 1AQM 1G94 1G9H 1JD7 1JD9
• 分子名称: ALPHA-AMYLASE, CALCIUM ION, NITRATE ION, ... (4 entities in total)
• 機能のキーワード: beta-alpha-8-barrel, 3 domain structure, hydrolase
• 由来する生物種: Pseudoalteromonas haloplanktis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 49049.51

構造登録者

Aghajari, N.,Haser, R. (登録日: 2002-02-12, 公開日: 2002-06-19, 最終更新日: 2024-10-30)

主引用文献

Aghajari, N.,Feller, G.,Gerday, C.,Haser, R.
Structural basis of alpha-amylase activation by chloride.
Protein Sci., 11:1435-1441, 2002

PubMed Abstract: To further investigate the mechanism and function of allosteric activation by chloride in some alpha-amylases, the structure of the bacterial alpha-amylase from the psychrophilic micro-organism Pseudoalteromonas haloplanktis in complex with nitrate has been solved at 2.1 A degrees, as well as the structure of the mutants Lys300Gln (2.5 A degrees ) and Lys300Arg (2.25 A degrees ). Nitrate binds strongly to alpha-amylase but is a weak activator. Mutation of the critical chloride ligand Lys300 into Gln results in a chloride-independent enzyme, whereas the mutation into Arg mimics the binding site as is found in animal alpha-amylases with, however, a lower affinity for chloride. These structures reveal that the triangular conformation of the chloride ligands and the nearly equatorial coordination allow the perfect accommodation of planar trigonal monovalent anions such as NO3-, explaining their unusual strong binding. It is also shown that a localized negative charge such as that of Cl-, rather than a delocalized charge as in the case of nitrate, is essential for maximal activation. The chloride-free mutant Lys300Gln indicates that chloride is not mandatory for the catalytic mechanism but strongly increases the reactivity at the active site. Disappearance of the putative catalytic water molecule in this weakly active mutant supports the view that chloride helps to polarize the hydrolytic water molecule and enhances the rate of the second step in the catalytic reaction.

PubMed: 12021442
DOI: 10.1110/ps.0202602

実験手法

X-RAY DIFFRACTION (2.1 Å)