1KYQ

Met8p: A bifunctional NAD-dependent dehydrogenase and ferrochelatase involved in siroheme synthesis.

Summary for 1KYQ

• Entry DOI: 10.2210/pdb1kyq/pdb
• Descriptor: Siroheme biosynthesis protein MET8, NICOTINAMIDE-ADENINE-DINUCLEOTIDE (3 entities in total)
• Functional Keywords: homodimer, oxidoreductase, lyase
• Biological source: Saccharomyces cerevisiae (baker's yeast)
• Total number of polymer chains: 3
• Total formula weight: 98858.08

Authors

Schubert, H.L.,Raux, E.,Brindley, A.A.,Wilson, K.S.,Hill, C.P.,Warren, M.J. (deposition date: 2002-02-05, release date: 2002-05-08, Last modification date: 2011-07-13)

Primary citation

Schubert, H.L.,Raux, E.,Brindley, A.A.,Leech, H.K.,Wilson, K.S.,Hill, C.P.,Warren, M.J.
The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase.
EMBO J., 21:2068-2075, 2002

PubMed Abstract: Sirohaem is a tetrapyrrole-derived prosthetic group that is required for the essential assimilation of sulfur and nitrogen into all living systems as part of the sulfite and nitrite reductase systems. The final two steps in the biosynthesis of sirohaem involve a beta-NAD(+)-dependent dehydrogenation of precorrin-2 to generate sirohydrochlorin followed by ferrochelation to yield sirohaem. In Saccharomyces cerevisiae, Met8p is a bifunctional enzyme that carries out both of these reactions. Here, we report the 2.2 A resolution crystal structure of Met8p, which adopts a novel fold that bears no resemblance to the previously determined structures of cobalt- or ferro-chelatases. Analysis of mutant proteins suggests that both catalytic activities share a single active site, and that Asp141 plays an essential role in both dehydrogenase and chelatase processes.

PubMed: 11980703
DOI: 10.1093/emboj/21.9.2068

Experimental method

X-RAY DIFFRACTION (2.2 Å)