1KYH

Structure of Bacillus subtilis YxkO, a Member of the UPF0031 Family and a Putative Kinase

1KYH の概要

• エントリーDOI: 10.2210/pdb1kyh/pdb
• 分子名称: Hypothetical 29.9 kDa protein in SIGY-CYDD intergenic region (2 entities in total)
• 機能のキーワード: structural genomics, psi, protein structure initiative, midwest center for structural genomics, mcsg, unknown function
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30185.55

構造登録者

Zhang, R.,Dementieva, I.,Vinokour, E.,Collart, F.,Joachimiak, A.,Midwest Center for Structural Genomics (MCSG) (登録日: 2002-02-04, 公開日: 2002-08-14, 最終更新日: 2024-10-30)

主引用文献

Zhang, R.,Grembecka, J.,Vinokour, E.,Collart, F.,Dementieva, I.,Minor, W.,Joachimiak, A.
Structure of Bacillus subtilis YXKO--a member of the UPF0031 family and a putative kinase.
J.Struct.Biol., 139:161-170, 2002

PubMed Abstract: We determined the 1.6-A resolution crystal structure of a conserved hypothetical 29.9-kDa protein from the SIGY-CYDD intergenic region encoded by a Bacillus subtilis open reading frame in the YXKO locus. YXKO homologues are broadly distributed and are by and large described as proteins with unknown function. The YXKO protein has an alpha/beta fold and shows high structural homology to the members of a ribokinase-like superfamily. However, YXKO is the only member of this superfamily known to form tetramers. Putative binding sites for adenosine triphosphate (ATP), a substrate, and Mg(2+)-binding sites were revealed in the structure of the protein, based on high structural similarity to ATP-dependent members of the superfamily. Two adjacent monomers contribute residues to the active site. The crystal structure provides valuable information about the YXKO protein's tertiary and quaternary structure, the biochemical function of YXKO and its homologues, and the evolution of its ribokinase-like superfamily.

PubMed: 12457846
DOI: 10.1016/S1047-8477(02)00532-4

実験手法

X-RAY DIFFRACTION (1.6 Å)